Fig. 7

Pb257 promotes P. brassicae infection in B. rapa. a Images of infected root hairs and epidermal cells at 7 dpi and 14 dpi, respectively, and symptoms at 30 dpi. The transient overexpression of Pb257 fused to the signal peptide (Pb257), host-induced gene silencing (HIGS) of Pb257 (Pb257-HIGS). b The disease index of B. rapa inoculated with P. brassicae. 50–60 seedlings in each treatment group were used for analysis. Significant difference was determined by Student’s t test. (** p < 0.01; *** p < 0.001). c qRT-PCR analysis of Pb257 transcript levels at different time points. The constitutively expressed gene PbActin was used as an internal reference. The experiment was replicated three times. d Detection of Pb257 expression levels by qRT-PCR at 8 days post-inoculation P. brassicae. Empty vector treatment was used as a control (CK), Pb257-O means transient overexpression of Pb257, Pb257-HIGS means the host-induced gene silencing (HIGS) of Pb257. Statistical analysis was performed by one-way ANOVA, followed by Tukey’s multiple comparison test. Error bars are the standard deviation (SD) of three independent replicates (* p < 0.05; ** p < 0.01)