Fig. 6From: Four sugarcane ScDIR genes contribute to lignin biosynthesis and disease resistance to Sporisorium scitamineumConstruction and verification of ScDIR transgenic sugarcane. a Agrobacterium tumefaciens cocultured with sugarcane explants. b Glufosinate ammonium containing 2.0 mg/L was used to screen transgenic-resistant sugarcane seedlings in the medium. c Rooting culture of resistant sugarcane seedlings. d The transgenic sugarcane lines. e qRT-PCR validation of transgenic sugarcane lines. The relative expression pattern of the gene was generated by Heml 1.0.3.7 software. The color code of the heatmap indicates the expression. GAPDH was used as an internal reference gene. Three biological replicates were carried out and each replicate includes three plantsBack to article page