Fig. 4
Truncated ScCDPK1 and ScCDPK8 significantly induced ScPR1 expression. a, c, e LUC signals generated in three sets of reporter assays by expressing ProScPR1:LUC and Pro35s:ScCDPK NT -HA (a ScCDPK1NT, ScCDPK2NT, and ScCDPK5NT; c ScCDPK4NT, ScCDPK6NT, and ScCDPK9NT; e ScCDPK7NT, ScCDPK8NT, and ScCDPK12NT). b, d, f Quantitative analysis of LUC signals generated in the assays depicted in a, c, and e, respectively. g Protein levels of ScCDPK1NT, ScCDPK2NT, ScCDPK4NT ScCDPK5NT, ScCDPK6NT, ScCDPK7NT, ScCDPK8NT, ScCDPK9NT, and ScCDPK12NT as indicated in Nicotiana benthamiana. As indicated, N. benthamiana leaves infiltrated with Agrobacterium strains carrying ProScPR1:LUC and Pro35s:ScCDPK.NT -HA were subjected to reporter assays. Infiltrated N. benthamiana leaves were sliced into strips 48 h post-infiltration, and relative luminescence was determined by a microplate luminometer. Error bars indicate SD of three biological repeats. Different letters indicate significant differences (P < 0.05, one-way ANOVA with Tukey’s multiple comparisons)