Fig. 5
Truncated ScCDPK1 and ScCDPK8 exhibited stronger activity than full-length ScCDPK1 and ScCDPK8 in inducing ScPR1 expression. a, c LUC signals generated in three sets of reporter assays by expressing ProScPR1:LUC with Pro35s:ScCDPK1 NT-HA, Pro35s:ScCDPK1 FL-HA, Pro35s:ScCDPK8 NT-HA, or Pro35s:ScCDPK8 FL-HA. b, d Quantitative analysis of LUC signals generated in the assays depicted in a and c. Agrobacteria carrying ProScPR1:LUC were infiltrated alone, or together with Agrobacteria carrying Pro35s:ScCDPK1 NT-HA, Pro35s:ScCDPK8 NT-HA, Pro35s:ScCDPK1 FL-HA, or Pro35s:ScCDPK8 FL-HA, as indicated. e Protein levels of ScCDPK1NT, ScCDPK1FL, ScCDPK8NT, and ScCDPK8.FL as indicated in N. benthamiana. Infiltrated N. benthamiana leaves were sliced into strips 48 h post-infiltration, and relative luminescence was determined by a microplate luminometer. Error bars indicate SD of three biological repeats. Different letters indicate significant differences (P < 0.05, one-way ANOVA with Tukey’s multiple comparisons)