Fig. 6
Truncated ScCDPK1 and ScDPK8 induced higher expression of ScPR1 and ScSPI than full-length ScCDPK1 and ScDPK8 in sugarcane protoplasts. a Relative levels of ScPR1 gene transcripts in protoplasts transfected with Pro35s:ScCDPK1 NT-HA, Pro35s:ScCDPK1 FL-HA, Pro35s:ScCDPK8 NT-HA, or Pro35s:ScCDPK8 FL-HA. b Relative levels of ScSPI gene transcripts in protoplasts transfected with Pro35s:ScCDPK1 NT-HA, Pro35s:ScCDPK1 FL-HA, Pro35s:ScCDPK8 NT -HA, or Pro35s:ScCDPK8 FL-HA. c Protein levels of ScCDPK1NT, ScCDPK1FL, ScCDPK8NT, and ScCDPK8FL as indicated in sugarcane protoplasts. Protoplasts isolated from Guitang 42 were transfected with Pro35s:ScCDPK1 FL-HA, Pro35s:ScCDPK8 FL-HA, Pro35s:ScCDPK1 NT-HA, or Pro35s:ScCDPK8.NT -HA. Total RNA was isolated from samples collected 8 h after treatments and used for cDNA synthesis followed by quantitative RT-PCR analysis of ScPR1 and ScSPI transcripts with specific primers. Error bars indicate SD of three biological repeats. Different letters indicate significant differences (P < 0.05, one-way ANOVA with Tukey’s multiple comparisons)