Fig. 6
Transient expression of AsCEP19 and AsCEP20 in N. benthamiana and tomato leaves. a The full-length (FL) AsCEP20 (AsCEP20FL) triggered obvious necrotic lesion at seven days post-infiltration. The GFP and INF1 plasmid constructs were infiltrated as negative and necrosis control, respectively. b AsCEP20FL elicited plant cell death in tomato leaves. c AsCEP19FL and AsCEP20FL promoted P. infestans infection in N. benthamiana. AsCEP19FL, AsCEP20FL, and GFP (empty vector) were individually agroinfiltrated into leaves for 48 h, and then the infiltrated leaves were detached from plants and inoculated with 10 μL zoospore suspension (5 × 104 zoospores/mL) of P. infestans. At 5 days post-inoculation (dpi), lesion size was measured, and the leaves were stained with DAB (Two-tailed Student's t test, * P < 0.05, ± SD, n = 6)