- Open Access
Maize catalases localized in peroxisomes support the replication of maize chlorotic mottle virus
Phytopathology Research volume 3, Article number: 17 (2021)
Co-infection of maize chlorotic mottle virus (MCMV) with a virus in the Potyviridae family, such as sugarcane mosaic virus, usually leads to maize lethal necrosis (MLN). Over the past decade, MCMV/MLN has emerged in many countries/regions of the world and resulted in serious yield loss in maize production. Although partial functions of some MCMV-encoded proteins have been identified, the host factors related to MCMV replication are poorly understood. Here, we show that maize peroxisomes can form aggregated bodies in MCMV-infected leaf cells. The dsRNA binding-dependent fluorescence complementation assay indicated that the aggregated peroxisomes in maize served as the major replication site of MCMV. In addition, our results revealed that all the three maize catalases were present mostly in peroxisomes in the presence or absence of MCMV. Furthermore, we determined that inhibition of catalase activity or induction of reactive oxygen species (ROS) in maize protoplasts significantly reduced the accumulation of MCMV RNA. In summary, this research reveals the replication site of MCMV and an important role of maize catalases in supporting virus replication. Our results are conducive to understanding the pathogenesis of MCMV and identifying targets for resistance breeding or gene regulation strategies.
Pathogenic infections in plants are frequently associated with the accumulation of reactive oxygen species (ROS), such as superoxide anion radical (.O2−), hydroxyl radical (.OH) and hydrogen peroxide (H2O2), which serve as signal transduction molecules to control a large array of biological processes, including senescence, growth and development, and biotic or abiotic stress responses (Schippers et al. 2012; Lehmann et al. 2015; Guo et al. 2017; Zhou et al. 2018). In photosynthetic cells, chloroplasts, mitochondria and peroxisomes are the major sites of intracellular ROS production (Mhamdi et al. 2010). The generation of ROS, known as the oxidative burst, is considered the earliest response of plant cells to biotic stimuli (Vranova et al. 2002). In addition, as a water-soluble, long half-life and non-radical two-electron reduction product of oxygen, H2O2 is generated as an important messenger to induce downstream defense proteins. Since increased ROS production during pathogen attack triggers accumulation of salicylic acid, induces pathogenesis-related protein genes and even cell death, the accumulation of ROS has been accepted as a hallmark of plant defense (Lamb and Dixon 1997; Dempsey et al. 1999). It is assumed that the generation of H2O2 at the correct time and location successfully hinders the penetration of pathogens into their host plants (Mellersh et al. 2002; Magbanua et al. 2007).
Although H2O2 is essential for signaling pathogen invasion and host defense, high level of H2O2 induces oxidative stress, ultimately resulting in cell and tissue death. To protect cells from the deleterious effects of H2O2, plants have evolved efficient enzymatic and non-enzymatic mechanisms to detoxify H2O2. Plants are capable of producing several types of enzymatic antioxidants (e.g. catalases and ascorbate peroxidases), peroxiredoxins, glutathione peroxidases and glutathione S-transferases to metabolize H2O2 (Anjum et al. 2016). Among the H2O2-scavenging enzymes, catalase (CAT) is a highly conserved enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) to produce water and oxygen and thus plays a critical role in plant disease resistance. CAT is a heme-containing enzyme that is found in diverse organisms, including prokaryotes, fungi, plants and animals (Chelikani et al. 2004). In higher plants, catalase is a tetrameric enzyme consisting of polypeptides of 50–70 kDa (Mhamdi et al. 2010).
In maize (Zea mays L.), the catalase gene family is composed of three members that are encoded by three unlinked genes (ZmCAT1, ZmCAT2 and ZmCAT3) (Roupakias et al. 1980). As in some higher plants, three catalases in maize share reasonably high sequence similarity at the amino acid level (63–77%) (Magbanua et al. 2007). Each of the catalase genes exhibits temporal and spatial specificity in its expression (Scandalios et al. 1984; Wadsworth and Scandalios 1989; Redinbaugh et al. 1990). For instance, ZmCAT1 is the only catalase isozyme expressed in milky endosperm, aleurone and scutellum during the early stages of kernel development (Guan and Scandalios 1993). ZmCAT2 is positively regulated by light in a tissue-specific manner, and the ZmCAT3 transcript shows a circadian rhythm, with a peak expression at the night/day transition (Skadsen and Scandalios 1987; Guan et al. 1996).
CAT is mainly localized in peroxisomes in higher plants. The non-cleaved tripeptide (S/T-R-L) at the extreme C-terminus of polypeptide have been identified for determining import of catalases into peroxisomes (Brown and Baker 2008; Mhamdi et al. 2010). ZmCAT1 and ZmCAT2 localize in the peroxisome (Abler and Scandalios 1993). ZmCAT3 might localize in the mitochondria since significant catalase activity (ZmCAT3) was detected in washed mitochondria (Scandalios et al. 1980). In addition, catalases play important roles in the interactions of host plants with viral pathogenicity-related proteins (Inaba et al. 2011; Mathioudakis et al. 2013; Murota et al. 2017; Roshan et al. 2018; Yang et al. 2020; Jiao et al. 2021). For instance, Arabidopsis thaliana catalase 3 interacts with cucumber mosaic virus (CMV-HL) 2b protein to inactivate the silencing suppressor activity of 2b (Inaba et al. 2011). However, whether catalases affect viral replication remains largely unknown.
Maize chlorotic mottle virus (MCMV) is the only member of the genus Machlomovirus in the family Tombusviridae, and its host range is restricted to Poaceae (Scheets 2016). The MCMV genome consists of a positive-sense single-stranded RNA of 4437 nt without a 5′ cap or a poly (A) tail, and encodes seven proteins including P32, P50, P111, P7a, P7b, P31 and coat protein (CP) (Nutter et al. 1989; Scheets et al. 1993; Scheets 2016) (Fig. 1a). P32 locates at a region proximal to the 5′ end of the viral genome, and is unique to MCMV in the family Tombusviridae (Nutter et al. 1989; Scheets 2016). The absence of P32 in MCMV dramatically decreases viral accumulation and symptoms in host plant (Scheets 2016). P50 and its readthrough protein P111 are replication proteins, similar with those in other viruses of the family Tombusviridae. Other four proteins (P7a, P31, P7b and CP) are expressed from subgenomic RNA1 (sgRNA1) (Scheets 2000, 2016). Mutagenesis analyses demonstrate that P7a, P7b and CP are required for cell-to-cell movement in maize plants (Scheets 2016). The MCMV CP interacts with the nuclear import factor importin-α for nuclear import, and silencing of the importin-α gene decreases MCMV accumulation (Zhan et al. 2016). Another unique protein P31 expressed as a readthrough extension of P7a is required for efficient systemic infection (Scheets 2016; Jiao et al. 2021). P31 is the major pathogenicity determinant of MCMV, and its expression induces necrosis in systemically infected leaves (Jiao et al. 2021). A 337-nt noncoding sgRNA2 was detected in MCMV-infected maize protoplasts and plants (Scheets 2000). MCMV causes chlorotic, mottle symptoms and even necrosis in maize plants. In addition, it is part of a synergistic disease called maize lethal necrosis (MLN) (Goldberg and Brakke 1987; Scheets 1998; Xia et al. 2016; Wang et al. 2017). MLN is caused by a mixed infection of MCMV and a virus in the Potyviridae family, such as sugarcane mosaic virus (SCMV) (genus Potyvirus) (Scheets 1998; Xia et al. 2016; Wang et al. 2017), maize dwarf mosaic virus (MDMV) (genus Potyvirus) (Goldberg and Brakke 1987), Johnsongrass mosaic virus (JGMV) (genus Potyvirus) (Stewart et al. 2017) and wheat streak mosaic virus (WSMV) (genus Tritimovirus) (Scheets 1998). MLN is a devastating disease which can cause up to 100% yield loss (Redinbaugh and Stewart 2018). Over the past decade, the emergence of MLN has resulted in significant economic losses in many maize-growing countries/regions of the world (Redinbaugh and Stewart 2018). Although efforts were made to identify resistance against MCMV, no genes conferring complete resistance have been reported (Redinbaugh and Stewart 2018; Carino et al. 2020). Some mechanisms underlying MCMV pathogenesis have been elucidated (Inaba et al. 2011; Scheets 2016; Zhan et al. 2016; Carino et al. 2020; Jiao et al. 2021), however, little is known about its replication mechanisms. In this research, we focused on the molecular mechanisms of MCMV replication, aiming at providing valuable knowledge towards identifying targets for virus resistance.
Here, we reveal that MCMV infection can induce the formation of aggregated bodies of peroxisomes in host plants. Our results demonstrate that all three maize catalases are localized in peroxisomes where MCMV replication occurs. In addition, inhibition of catalase activity negatively regulates MCMV replication. This study contributes to our understanding of MCMV replication and is helpful for controlling MLN.
MCMV infection induces the formation of aggregated bodies of peroxisomes in maize leaves
Here, 8-day-old maize seedlings (B73) inoculated with MCMV (pMCM41) showed chlorotic, mottle symptoms and even necrosis at 10 days post-inoculation (dpi), whereas no symptom was observed in control plants mock-inoculated with phosphate buffer (Fig. 1b, c). Our previous research revealed that peroxisomes were highly aggregated in MCMV-infected maize leaves (Jiao et al. 2021). To further determine whether a similar localization pattern of peroxisomes occurs in maize living cells after MCMV infection, we used DsRed-SKL (a peroxisome marker) to visualize the distribution of peroxisomes. Gold particles coated with pGD-DsRed or pGD-DsRed-SKL were bombarded into mock- and MCMV-inoculated maize (B73) leaves. Red fluorescence was observed at 36 h post-bombardment (hpb). The fluorescence for DsRed alone was distributed in both the cytoplasm and nucleus (Fig. 2a). Punctate red fluorescence in the cytoplasm was observed when DsRed-SKL was expressed in mock-inoculated maize leaves (Fig. 2a). Intriguingly, red fluorescence from DsRed-SKL was redistributed to aggregated bodies in MCMV-infected maize leaves (Fig. 2b), while the fluorescence for DsRed alone was unchanged after MCMV infection (Fig. 2b). These results indicate that MCMV infection induces maize peroxisomes to form aggregated bodies.
Aggregated peroxisomes serve as the replication sites of MCMV
It was reported that the aggregated peroxisomes in plants serve as the viral replication sites during infection by tomato bushy stunt virus (TBSV) (McCartney et al. 2005). We presumed that MCMV infection might also cause aggregation of peroxisomes to facilitate its replication in host cells. MCMV encodes two replication proteins, P50 and P111, which are necessary for virus replication (Scheets 2016). To validate whether MCMV RNA synthesis takes place in the aggregated peroxisomes, maize protoplasts were co-transfected with the B2-YN/VP35-YC reporter, GFP-P50 and MCMV in vitro transcripts. Since B2-YN and VP35-YC specifically bind to dsRNA, the reconstituted YFP fluorescence indicates their co-binding to MCMV double-stranded replicative intermediates (Cheng et al. 2015). Confocal results showed that the dsRNA detected by B2-YN and VP35-YC colocalized with the MCMV replication protein P50 (Fig. 3a). No YFP fluorescence signal was observed when maize protoplasts were co-transfected with the B2-YN/VP35-YC reporter and GFP-P50 in the absence of MCMV (Fig. 3b). Thus, P50 could be used as a marker for identifying the viral replication complex (VRC) of MCMV. We subsequently transfected maize protoplasts with GFP-P50, DsRed-SKL and MCMV in vitro transcripts. As shown in Fig. 3c, GFP-P50 was closely associated with the aggregated peroxisomes (DsRed-SKL). In addition, the P50 protein, expressed alone, targeted and induced maize peroxisomes to form aggregated bodies (Fig. 3d). These results indicate that aggregated peroxisomes serve as the replication sites of MCMV.
ZmCATs are localized in peroxisomes in maize cells
Although CAT is known to localize primarily in the peroxisomes in many plants, the localization of maize catalases remains largely unknown (Abler and Scandalios 1993). To determine the subcellular localization of ZmCATs, maize protoplasts were co-transfected with pGD-GFP-ZmCAT1/2/3 or pGD-GFP and DsRed-SKL. Fluorescence in maize protoplasts expressing GFP was observed in both the cytoplasm and nucleus (Fig. 4a, line 1), whereas fluorescence from GFP-ZmCATs colocalized with the red fluorescence produced by DsRed-SKL (Fig. 4a, lines 2–4). Similar results were observed in maize epidermal cells (Fig. 4b). A previous study reported that ZmCAT3 might be localized in mitochondria (Willekens et al. 1995). Here, we examined the localization of GFP-ZmCAT3 in maize protoplasts with mitochondria-specific dye Mito-Tracker Red, and found that the GFP fluorescent signal did not co-localize with Mito-Tracker Red (Additional file 1: Figure S1). These data indicate that all three ZmCATs are localized in the peroxisomes in maize cells.
ZmCATs are localized in aggregated peroxisomes in the presence of MCMV
To explore whether the subcellular localization of ZmCATs is altered during MCMV infection, maize protoplasts were co-transfected with pGD-GFP-ZmCAT1/2/3 and in vitro transcripts of MCMV. Green fluorescence was observed at 16–24 h post-transfection (hpt). Interestingly, green fluorescence (GFP-ZmCATs) was observed to form aggregated bodies in the cytoplasm during MCMV infection (Fig. 5a, lanes 2–4), whereas no such aggregated bodies were detected in healthy maize cells (Fig. 5a, lane 1). In addition, similar results were obtained when GFP-ZmCAT1/2/3 were introduced into MCMV-inoculated leaf cells by particle bombardment (Fig. 5b). When GFP-ZmCAT1, DsRed-SKL and in vitro transcripts of MCMV were transfected together into maize protoplasts, GFP-ZmCAT1 colocalized with aggregated DsRed-SKL (Fig. 5c). Similar to the results obtained in maize protoplasts, the fluorescence of GFP-ZmCAT1 remained in the aggregated bodies formed by DsRed-SKL in maize epidermal cells (Fig. 5d). We confirmed the accumulation of MCMV in maize tissues used for bombardment by immunoblotting (Additional file 1: Figure S2). These results indicate that maize catalases are localized in aggregated peroxisomes during MCMV infection. Since MCMV carries out its replication in aggregated peroxisomes, we presumed that catalases play a role in viral replication. To investigate the possible role of catalase in MCMV replication, maize protoplasts were transfected with in vitro transcripts of MCMV and then incubated with 20 mM 3-amino-1, 2, 4-triazole (3-AT, a catalase inhibitor). Northern blot analyses were subsequently conducted with RNA extracts at 18 hpt to quantify the accumulation of MCMV RNA. As shown in Fig. 5e, treatment with 3-AT negatively regulated MCMV RNA accumulation. Catalase can protect cells from the deleterious effects of H2O2, and the decreased catalase activity may lead to increased accumulation of H2O2 to produce negative effect on MCMV replication. Importantly, high intracellular ROS production was observed when maize protoplasts were treated with the ROS inducer methyl viologen (MV) (Additional file 1: Figure S3). Thus, maize protoplasts were transfected with in vitro transcripts of MCMV and then incubated with MV for 18 h. Northern blot analyses indicated that treatment of maize protoplasts with MV led to inhibition of MCMV replication (Fig. 5e). Together, these results suggest that maize catalase activity plays an important role in supporting MCMV replication.
P50 and its readthrough protein P111 are the only MCMV proteins required for replication (Scheets 2016), however, the precise replication site of MCMV remains obscure. Using the in vivo double-stranded RNA (dsRNA) labeling system, we showed that the replication of MCMV is associated with peroxisomes (Fig. 3). MCMV infection caused aggregation of peroxisomes (Figs. 2 and 3), with similar situations occurring in infections by some viruses in the family of Tombusviridae (Cook et al. 2019). For instance, tomato bushy stunt virus (TBSV), cucumber necrosis virus (CNV) and cymbidium ringspot virus (CymRSV) use peroxisomes as scaffolds for viral replication factories (Cook et al. 2019). Upon infection, the TBSV p33 protein targets initially from the cytosol to peroxisomes to eventually generate the peroxisome-derived multivesicular bodies (McCartney et al. 2005). During CNV infection, the peroxisome biogenesis is enhanced to facilitate virus replication (Rochon et al. 2014). In addition to support the replication of plant viruses, peroxisomes have long been recognized as key players in human virus infections. For instance, human cytomegalovirus (HCMV) and herpes simplex virus type 1 (HSV-1) change peroxisome morphology to support their replication (Jean Beltran et al. 2018). Additionally, some flaviviruses such as dengue virus (DENV) and West Nile virus (WNV) target their capsid proteins to peroxisome membranes, resulting in a significant decrease in the number of peroxisomes and consequently inhibiting type III interferon expression (peroxisome-mediated immune signaling) (You et al. 2015). On the one hand, studying viral infection provides the opportunity for addressing core questions of peroxisome biology. On the other hand, elucidating the processes underlying peroxisome biology may also provide strategies to selectively inhibit peroxisome-mediated functions required for viral infection.
In this study, we determined that all the three maize catalases are localized in peroxisomes (Fig. 4). Both ZmCAT1 and ZmCAT2 contain a tripeptide SRL (while ZmCAT3 contains a TRL) with a peroxisomal targeting signal in the C-terminal. Previous study reported that ZmCAT3 co-isolated with the mitochondrial fraction of mesophyll cells (Scandalios et al. 1980). In addition, proteomic analysis of highly purified mitochondria from Arabidopsis cells also identified AtCAT2 and AtCAT3 (Heazlewood et al. 2004). There has been no demonstration of catalase import into angiosperm mitochondria. These findings may indicate a progressive decline throughout the mitochondrial purification procedure. Thus, it is possible that contamination may account for previous reports on catalase activity in mitochondria. However, peroxisomal catalase can change the localization to other organelles under some conditions. For instance, yeast catalase was co-localized to mitochondria in a manner dependent on nutritional conditions (Petrova et al. 2004; Mhamdi et al. 2010). Therefore, dual peroxisomal/mitochondrial targeting of catalases cannot be ruled out. In this study, the catalase activity is required for MCMV replication because treatment with catalase inhibitor 3-AT reduced MCMV replication (Fig. 5e). Since the accumulation of H2O2 can impede virus replication, it is likely that catalases are needed to maintain a specific site for assembling viral replication complex (VRC) (Fig. 5e). This may also explain why the localization of ZmCATs to peroxisome is not altered in the presence of MCMV.
CAT is not only a strong antioxidant enzyme in scavenging H2O2, but also plays critical roles in plant responses to various stresses. It is reported that a rice salt tolerance receptor-like cytoplasmic kinase 1 (STRK1) anchors and interacts with OsCatC at the plasma membrane (Zhou et al. 2018). The OsCatC is phosphorylated and activated by STRK1, thereby regulating H2O2 homeostasis and improving salt tolerance in rice (Zhou et al. 2018). Recently, evidence has emerged that AtCAT2 regulates leaf senescence in Arabidopsis (Guo et al. 2017). The transcription factor WRKY75 as a positive regulator of leaf senescence suppresses H2O2 scavenging partly by repressing the transcription of AtCAT2 (Guo et al. 2017). Increasing evidences show that catalase regulates plant resistance to diverse pathogens. For instance, biotrophic-pathogen-induced salicylic acid (SA) accumulation dampens both auxin and jasmonic acid (JA) synthesis by inhibiting AtCAT2 activity in Arabidopsis (Yuan et al. 2017). Suppression of AtCAT2 by SA results in high levels of H2O2, leading to sulfenylation of tryptophan synthetase b subunit 1, thus depleting the auxin biosynthetic precursor tryptophan (Yuan et al. 2017). AtCAT2 promotes acyl-CoA oxidase 2 (ACX2) and ACX3 activity to increase JA accumulation (Yuan et al. 2017). In addition, the pathogenic oomycete Phytophthora sojae secretes two cytoplasmic effectors, PsCRN63 and PsCRN115, to recruit NbCAT1 into nucleus (Zhang et al. 2015). Furthermore, these two effectors manipulate plant programmed cell death (PCD) through interfering with catalases and eventually perturbing H2O2 homeostasis (Zhang et al. 2015).
The effects of interactions between CATs and viruses varied in different host-pathogen systems. For instance, helper component proteinase (HC-Pro) encoded by chilli veinal mottle virus (ChiVMV) directly interacts with CAT1 and CAT3 of Nicotiana tabacum in the cytoplasm to facilitate viral infection (Yang et al. 2020). Neither CAT1 nor CAT3 affects the RNA silencing suppression activity of HC-Pro (Yang et al. 2020). However, the interaction between CMV-HL 2b and AtCAT3 significantly inactivates the RNA silencing suppression activity of CMV-HL 2b (Inaba et al. 2011). Intriguingly, some virus-induced necrosis are consequences of direct protein-protein interactions between viral pathogenicity determinants and host catalases. In our previous study, we reported that MCMV P31 interacts with maize catalases 1/3 to inhibit their catalase activities, and subsequently attenuate SA-mediated defense (Jiao et al. 2021). Thus, systemic necrosis is observed in upper non-inoculated leaves (Jiao et al. 2021). Similarly, tomato leaf curl Palampur virus AV2 protein promotes systemic necrosis in Nicotiana benthamiana plants by interacting with host CAT2 (Roshan et al. 2018). In this study, we revealed that catalase activity positively regulates MCMV replication especially under high-ROS condition (Fig. 5e). Maize catalases have been identified to regulate several processes in MCMV infection. The regulatory mechanisms underlying the ROS metabolism and MCMV infection need to be further investigated.
MCMV is the emerging virus that drives MLN expansion. Elucidating the molecular mechanisms underlying MCMV pathogenesis will be conducive to controlling this devastating disease. In this study, we revealed that MCMV infection induced maize peroxisomes to form aggregated bodies, which served as viral replication sites. The three maize catalases were shown to localize in peroxisomes in the absence and presence of MCMV RNA. In addition, inhibiting catalase activity negatively regulated MCMV replication. The results are conducive to elucidating the replication mechanisms of MCMV, and also to understanding the molecular interaction between MCMV and host maize.
Plant growth conditions and virus inoculation
Maize seedlings (inbred line B73) were grown in a growth chamber (24 °C day/22 °C night, 16 h light/8 h dark) for virus inoculation and analyses.
MCMV was prepared from the full-length cDNA clone (pMCM41) (Scheets et al. 1993). Crude extracts were prepared by homogenizing the MCMV-infected maize (B73) leaf tissues in 0.01 M phosphate buffer [0.01 M KH2PO4: 0.01 M Na2HPO4 = 49:51 (v/v), pH 7.0] at 1:10 (w/v) ratio.
The coding sequences of ZmCAT1 (GenBank accession no. NM_001254879.2), ZmCAT2 (NM_001111840.2) and ZmCAT3 (NM_001363892.1) were amplified individually from maize by reverse transcription-PCR (RT-PCR). For transient expression assays, constructs were based on a pGD vector (Goodin et al. 2002). All constructs were confirmed by sequencing. Primers used in this study are listed in Additional file 2: Table S1.
Preparation of MCMV RNA transcripts
RNA transcripts of MCMV (pMCM41) were prepared as described previously (Scheets et al. 1993; Zhu et al. 2014). Plasmids (pMCM41) linearized with Sma I were used for in vitro transcription of RNAs. The MCMV RNA transcripts were synthesized using a T7 RiboMAX™ Express Large Scale RNA Production System (Promaga, Madison, WI, USA; Cat: P1320). Then the RNA transcripts were treated with RQ1 RNase-free DNase (Promega) followed by extraction with phenol/chloroform and then only chloroform. Next, the resulting supernatant was mixed with 3 M NaAc (pH 5.2) (10:1, v/v) and ethanol (1:2, v/v) followed by precipitation at -80 °C for 20 min. The RNA transcripts were pelleted at 13000 g for 10 min at 4 °C and the pellet was washed twice with ice-cold 75% ethanol (1 mL). The pellet was then resuspended in RNase-free double-distilled (dd)H2O.
Isolation and transfection of maize protoplasts
Isolation and transfection of maize protoplasts were conducted as described previously (Sheen 1991; Zhu et al. 2014). For catalase inhibitor treatment assay, maize protoplasts were transfected with in vitro transcripts of MCMV (1 μg) and incubated with 20 mM 3-amino-1, 2, 4-triazole (3-AT; Sigma-Aldrich) at 25 °C under light for 18 h. For the ROS inducer methyl viologen (MV) treatment assay, maize protoplasts were transfected with in vitro transcripts of MCMV (1 μg) and incubated with 10 μM MV (Sigma-Aldrich) at 25 °C under light for 18 h. Each experiment was repeated at least three times using different batches of maize protoplasts.
Particle bombardment was performed as described previously (Kirienko et al. 2012; Chen et al. 2017), with slight modifications. One-month-old maize plants (B73) were inoculated with MCMV or phosphate buffer (mock). At 2 weeks post-inoculation, the maize tissue samples were collected and prepared before use. Approximately 8 μL (60 μg/μL) of the gold particle stock solution was mixed with 5 μg plasmid DNA, 20 μL of 0.1 M spermidine solution and 10 μL of 2.5 M CaCl2 solution in an Eppendorf tube. The mixture was vortexed at a low speed for 3 min and then pelleted at 10,000 g for 20 s. After two washes in 1 mL of 100% ethanol, the pellets of gold-plasmid DNA particles were resuspended with 10 μL of 100% ethanol solution and transferred to microcarriers (Bio-Rad). Vacuum pressure within the chamber was allowed to reach 27 to 28 inches of mercury prior to firing (PDS-1000/He system, Bio-Rad). The bombarded leaves were allowed to recover at 25 °C in the dark and examined for the expression of fusion proteins at 36 hpb.
dsRNA binding-dependent fluorescence complementation (dRBFC)
Mazie protoplasts were prepared and transfected with a previously reported B2-YN/VP35-YC reporter (Cheng et al. 2015) and GFP-P50. At 3 dpt, the protoplasts were harvested for imaging with a confocal microscope.
Detection of ROS production
For ROS staining assays with CM-H2DCFDA [5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate; Invitrogen], maize protoplasts were incubated with the ROS inducer MV for 18 h. Then the maize protoplasts were incubated for 15 min at room temperature in 1 μM CM-H2DCFDA. Fluorescence was observed by using a laser scanning confocal microscope (Leica, SP8).
Confocal microscopic observation
For subcellular localization and dRBFC assays, samples were observed under a laser scanning confocal microscope (Leica, SP8). The fluorescence of GFP and YFP was both excited at 488 nm, then captured at 495–545 nm and 520–580 nm, respectively. The fluorescence of DsRed were excited by wavelength of 552 nm and detected at 562 to 620 nm. For co-localization analysis, each of the channel was scanned and imaged sequentially. The images were processed using Adobe Photoshop (Adobe, San Jose, CA, USA).
The protein extraction and immunoblot assays were performed as described previously (Cao et al. 2012). MCMV coat protein (CP) antibody was kindly provided by Profs. Xueping Zhou and Jianxiang Wu (Zhejiang University) and used at a dilution of 1: 10000.
Availability of data and materials
3-amino-1, 2, 4-triazole
Chilli veinal mottle virus
Cucumber mosaic virus
dsRNA binding-dependent fluorescence complementation
Green fluorescent protein
- H2O2 :
Herpes simplex virus type 1
Johnsongrass mosaic virus
Maize chlorotic mottle virus
Maize dwarf mosaic virus
Maize lethal necrosis
- .O2 − :
Superoxide anion radical
Programmed cell death
Reactive oxygen species
Sugarcane mosaic virus
Salt tolerance receptor-like cytoplasmic kinase 1
Tomato bushy stunt virus
Virus replication complex
West Nile virus
Wheat streak mosaic virus
Yellow fluorescent protein
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We thank Dr. Kay Scheets (Oklahoma State University) for providing the full-length cDNA clone of MCMV (pMCM41), Dr. Andrew Jackson (University of California, Berkeley) for providing the pGD vector, Dr. Aiming Wang (Agriculture and Agri-Food Canada) for providing the dRBFC plasmids. We are indebted to Drs. Xueping Zhou and Jianxiang Wu (Zhejiang University) for providing the DsRed-SKL vector and MCMV CP antibody. We thank Drs. Na Jiang and Linlu Qi (China Agricultural University) for confocal microscopy assistance.
This work was supported by grants from the Ministry of Science and Technology (2016YFD0300710), the National Natural Science Foundation of China (no. 31571980), the Ministry of Education (the 111 Project B13006), and State Key Laboratory of Agrobiotechnology (2019SKLAB6–19).
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Additional file 1: Figure S1.
Subcellular localization of ZmCAT3 in maize protoplasts. Mazie protoplasts were transfected with pGD-GFP-ZmCAT3 for 18 h. Mito-Tracker Red was retained in the mitochondria before photographing. Bars, 10 μm. Figure S2. The accumulation of maize chlorotic mottle virus (MCMV) coat protein (CP) in tissues used for bombardment. Equal amounts of total proteins extracted from MCMV-infected maize (B73) leaves (positive control), phosphate buffer (mock)- or MCMV-inoculated maize (B73) tissues (to be used for bombardment, i.e. pGD-GFP-ZmCAT1/2/3, etc.) were used for immunoblot analysis with MCMV CP antibody. Figure S3 The H2DCFDA-stained maize protoplasts in the presence of methyl viologen (MV). a, b Maize protoplasts were treated with ddH2O (control, a) or 10 μM ROS inducer MV (b) for 18 h. The maize protoplasts were incubated for 15 min at room temperature in 1 μM CM-H2DCFDA. Fluorescence was observed by using a laser scanning confocal microscope. Bars, 100 μm.
Additional file 2: Table S1.
Primers used for construction of plasmids and the probes for Northern blotting of MCMV genomic RNAs.
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Jiao, Z., Wang, J., Tian, Y. et al. Maize catalases localized in peroxisomes support the replication of maize chlorotic mottle virus. Phytopathol Res 3, 17 (2021). https://doi.org/10.1186/s42483-021-00094-x
- Maize chlorotic mottle virus (MCMV)
- Replication site
- Virus replication